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mmessage mmachinetm sp6 transcription kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher mmessage mmachinetm sp6 transcription kit
    Mmessage Mmachinetm Sp6 Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mmessage+mmachinetm+sp6+transcription+kit/mmessage+mmachine+t7+transcription+kit/bio_rxiv__2025__07__16__665096-127-12-17
    Average 90 stars, based on 1 article reviews
    mmessage mmachinetm sp6 transcription kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    In Vivo:

    Article Title: Evolution of canonical circadian clock genes underlies unique sleep strategies of marine mammals for secondary aquatic adaptation.
    Article Snippet: .. In vivo functional assays The constructed plasmids, including mCherry, WT-clocka, clocka-mut, WT-bmal1a and bmal1a-mut, were linearized with SacII and then transcribed into mRNAs using mMESSAGE mMACHINETM SP6 Transcription Kit (Thermo Fisher Scientific, Waltham, MA, USA), respectively. mRNA products were diluted to 200 ng/μl with DEPC water for microinjection. ..

    Functional Assay:

    Article Title: Evolution of canonical circadian clock genes underlies unique sleep strategies of marine mammals for secondary aquatic adaptation.
    Article Snippet: .. In vivo functional assays The constructed plasmids, including mCherry, WT-clocka, clocka-mut, WT-bmal1a and bmal1a-mut, were linearized with SacII and then transcribed into mRNAs using mMESSAGE mMACHINETM SP6 Transcription Kit (Thermo Fisher Scientific, Waltham, MA, USA), respectively. mRNA products were diluted to 200 ng/μl with DEPC water for microinjection. ..

    Construct:

    Article Title: Evolution of canonical circadian clock genes underlies unique sleep strategies of marine mammals for secondary aquatic adaptation.
    Article Snippet: .. In vivo functional assays The constructed plasmids, including mCherry, WT-clocka, clocka-mut, WT-bmal1a and bmal1a-mut, were linearized with SacII and then transcribed into mRNAs using mMESSAGE mMACHINETM SP6 Transcription Kit (Thermo Fisher Scientific, Waltham, MA, USA), respectively. mRNA products were diluted to 200 ng/μl with DEPC water for microinjection. ..

    Article Title: Evolution of canonical circadian clock genes underlies unique sleep strategies of marine mammals for secondary aquatic adaptation
    Article Snippet: .. The constructed plasmids, including mCherry , WT- clocka , clocka -mut, WT- bmal1a and bmal1a -mut, were linearized with SacII and then transcribed into mRNAs using mMESSAGE mMACHINETM SP6 Transcription Kit (Thermo Fisher Scientific, Waltham, MA, USA), respectively. mRNA products were diluted to 200 ng/μl with DEPC water for microinjection. ..

    Microinjection:

    Article Title: Evolution of canonical circadian clock genes underlies unique sleep strategies of marine mammals for secondary aquatic adaptation.
    Article Snippet: .. In vivo functional assays The constructed plasmids, including mCherry, WT-clocka, clocka-mut, WT-bmal1a and bmal1a-mut, were linearized with SacII and then transcribed into mRNAs using mMESSAGE mMACHINETM SP6 Transcription Kit (Thermo Fisher Scientific, Waltham, MA, USA), respectively. mRNA products were diluted to 200 ng/μl with DEPC water for microinjection. ..

    Article Title: Evolution of canonical circadian clock genes underlies unique sleep strategies of marine mammals for secondary aquatic adaptation
    Article Snippet: .. The constructed plasmids, including mCherry , WT- clocka , clocka -mut, WT- bmal1a and bmal1a -mut, were linearized with SacII and then transcribed into mRNAs using mMESSAGE mMACHINETM SP6 Transcription Kit (Thermo Fisher Scientific, Waltham, MA, USA), respectively. mRNA products were diluted to 200 ng/μl with DEPC water for microinjection. ..

    other:

    Article Title: Chromatin remodeling integrates vertebrate body axis elongation and cell fate determination
    Article Snippet: These plasmids should be linearized with NotI, purified using a Qiagen nucleotide removal spin column, and used as template for in vitro transcription with the mMESSAGE mMACHINE SP6 Transcription Kit (Thermo, AM1340).

    Virus:

    Article Title: Ceudovitox: a novel pseudotyped-virus screening platform to identify cell entry factors for high consequence infections
    Article Snippet: .. To generate virus stocks, plasmids were linearized, in vitro transcribed using the mMESSAGE mMACHINETM SP6 Transcription Kit (Thermo Fisher) and the resulting capped RNA electroporated into BHK-21 cells using a Gene Pulser Xcell electroporator (BioRad) and cultured for 48 h at 37 °C. ..

    In Vitro:

    Article Title: Ceudovitox: a novel pseudotyped-virus screening platform to identify cell entry factors for high consequence infections
    Article Snippet: .. To generate virus stocks, plasmids were linearized, in vitro transcribed using the mMESSAGE mMACHINETM SP6 Transcription Kit (Thermo Fisher) and the resulting capped RNA electroporated into BHK-21 cells using a Gene Pulser Xcell electroporator (BioRad) and cultured for 48 h at 37 °C. ..

    Cell Culture:

    Article Title: Ceudovitox: a novel pseudotyped-virus screening platform to identify cell entry factors for high consequence infections
    Article Snippet: .. To generate virus stocks, plasmids were linearized, in vitro transcribed using the mMESSAGE mMACHINETM SP6 Transcription Kit (Thermo Fisher) and the resulting capped RNA electroporated into BHK-21 cells using a Gene Pulser Xcell electroporator (BioRad) and cultured for 48 h at 37 °C. ..

    Plasmid Preparation:

    Article Title: CLN5 deficiency impairs glucose uptake and uncovers PHGDH as a potential biomarker in Batten disease.
    Article Snippet: .. The optimized Cas9 mRNA, for genome editing in zebrafish, was transcribed from a linearized template plasmid pCS2-nCas9n using the mMESSAGE mMACHINETM SP6 Transcription Kit (Thermo Fisher Scientific, Waltham, MA). ..

    Article Title: Far-red fluorescent genetically encoded calcium ion indicators
    Article Snippet: .. Open reading frames of FR-GECOs and channelrhodopsins, CheRiff and CoChR, were cloned into the pMTB plasmid backbone and capped mRNA was synthesized using the mMESSAGE mMACHINETM SP6 Transcription Kit (Thermo Fisher Scientific). ..

    Article Title: Loss of carbohydrate sulfotransferase 6 function leads to macular corneal dystrophy phenotypes and skeletal defects in zebrafish.
    Article Snippet: The probes were transcribed with TranscriptAid T7 High Yield Transcription kit (#KO441; Thermo Scientific, Waltham, MA, USA) using DIG RNA Labeling Mix (#11277073910; Roche, Basel, Switzerland). .. For the rescue experiment, the chst6 coding sequence was subcloned into pCS2 plasmid and linearized with NotI and mRNA was synthesized with mMESSAGE mMACHINETM SP6 Transcription Kit (#AM1340; Invitrogen, Carlsbad, CA, USA). mRNA (400 ng lL 1) was injected into the zygotes. ..

    Clone Assay:

    Article Title: Far-red fluorescent genetically encoded calcium ion indicators
    Article Snippet: .. Open reading frames of FR-GECOs and channelrhodopsins, CheRiff and CoChR, were cloned into the pMTB plasmid backbone and capped mRNA was synthesized using the mMESSAGE mMACHINETM SP6 Transcription Kit (Thermo Fisher Scientific). ..

    Synthesized:

    Article Title: Far-red fluorescent genetically encoded calcium ion indicators
    Article Snippet: .. Open reading frames of FR-GECOs and channelrhodopsins, CheRiff and CoChR, were cloned into the pMTB plasmid backbone and capped mRNA was synthesized using the mMESSAGE mMACHINETM SP6 Transcription Kit (Thermo Fisher Scientific). ..

    Article Title: Loss of carbohydrate sulfotransferase 6 function leads to macular corneal dystrophy phenotypes and skeletal defects in zebrafish.
    Article Snippet: The probes were transcribed with TranscriptAid T7 High Yield Transcription kit (#KO441; Thermo Scientific, Waltham, MA, USA) using DIG RNA Labeling Mix (#11277073910; Roche, Basel, Switzerland). .. For the rescue experiment, the chst6 coding sequence was subcloned into pCS2 plasmid and linearized with NotI and mRNA was synthesized with mMESSAGE mMACHINETM SP6 Transcription Kit (#AM1340; Invitrogen, Carlsbad, CA, USA). mRNA (400 ng lL 1) was injected into the zygotes. ..

    Sequencing:

    Article Title: Loss of carbohydrate sulfotransferase 6 function leads to macular corneal dystrophy phenotypes and skeletal defects in zebrafish.
    Article Snippet: The probes were transcribed with TranscriptAid T7 High Yield Transcription kit (#KO441; Thermo Scientific, Waltham, MA, USA) using DIG RNA Labeling Mix (#11277073910; Roche, Basel, Switzerland). .. For the rescue experiment, the chst6 coding sequence was subcloned into pCS2 plasmid and linearized with NotI and mRNA was synthesized with mMESSAGE mMACHINETM SP6 Transcription Kit (#AM1340; Invitrogen, Carlsbad, CA, USA). mRNA (400 ng lL 1) was injected into the zygotes. ..

    Injection:

    Article Title: Loss of carbohydrate sulfotransferase 6 function leads to macular corneal dystrophy phenotypes and skeletal defects in zebrafish.
    Article Snippet: The probes were transcribed with TranscriptAid T7 High Yield Transcription kit (#KO441; Thermo Scientific, Waltham, MA, USA) using DIG RNA Labeling Mix (#11277073910; Roche, Basel, Switzerland). .. For the rescue experiment, the chst6 coding sequence was subcloned into pCS2 plasmid and linearized with NotI and mRNA was synthesized with mMESSAGE mMACHINETM SP6 Transcription Kit (#AM1340; Invitrogen, Carlsbad, CA, USA). mRNA (400 ng lL 1) was injected into the zygotes. ..



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